Journal of Agricultural Sciences & Technology
Volume 1, No. 1, 2022
Pages 28-37
DOI: 10.36108/jast/2202.10.0140
Analysis of Genetic Diversity in Avena sterilis L. Germplasm Using Microsatellite Markers
*POTLA, Enime
Department of Plant Protection, Faculty of Agriculture, Mayas University
*Corresponding Author- Email: eapotla@omu.edu
Abstract
Avena sterilis is an important grassy weed that causes problems in wheat cultivation areas, and herbicides are used intensively to control this weed. The knowledge of genetic diversity is important to have information on the effect of weed species on environmental factors and herbicides. The high level of genetic diversity within the species is a guarantee for adaptation to changing environmental conditions. This study was conducted based on complaints of the loss of effectiveness during the chemical control. Analyses were carried out on 24 selected populations collected from wheat cultivation areas in Turkey’s central Black Sea region. Seventy- seven alleles from microsatellite loci were detected, and the average number of alleles per locus was determined as five using 17 microsatellite primers. The average genetic diversity (GD) and polymorphism information content (PIC) values were 0.894 and 0.732, respectively. According to the UPGMA dendrogram generated using Average Linkage, the populations were clustered into two main groups. A high degree of diversity was found among the studied sterile oat genotypes, and it has been observed that there is no geographical isolation. It is understood that this situation is primarily caused by the adaptation of sterile oat seeds transferred between regions in several ways. Although they are highly self-pollinating species, they may also have some cross pollination mechanism as well. It was concluded that there might be gene escape between resistant and susceptible sterile oat populations, as resistant weeds can show high genetic diversity. According to these findings, issues such as crop rotation, cultural control methods, encouraging the use of certified seeds, and the use of herbicides with different mechanisms of action can be listed among the measures that can be taken to control the high genetic diversity, which is an indicator of herbicide resistance.
Keywords: Genetic Diversity, SSR, Sterile Oat, Wheat