Journal of Agricultural Sciences & Technology
Volume 1, No. 1, 2022
Pages 1-7

DOI: 10.36108/jast/2202.10.0110

Bioactive components of Erythrophleum suaveolens (Brenan) leaves extracts and their antifungal effect on Aspergillus flavus isolated from maize seeds

*YUSUF, Hajara Oyiza ¹, OLU, Joshua ², SOLEBO-AJENIFUJAH, Shakirat O.³, PETU-IBIKUNLE, Abiona Michael⁴, ALU, Joy Adaeze⁵

¹Environmental Biotechnology and Bio conservation Department, National Biotechnology Development Agency,  Along Musa Yar’adua Expressway, Airport Road, Lugbe, Abuja
²Faculty of Environmental Science, Nasarawa State University, Keffi, Nigeria
³Plant Biotechnology Lab, Agricultural Biotechnology Department, National Biotechnology Development Agency along Musa Yar’adua Expressway, Airport Road, Lugbe –Abuja

⁴Faculty of Agricultural Science, National Open University of Nigeria, Abuja
⁵Crop Science Laboratory, Faculty of Agriculture, University of Abuja

*Corresponding Author- Email: haj4yusuf@gmail.com          Orcid No: http://orcid,org/0000-0003-4296-6674

Abstract

This study set out to evaluate the phytochemical composition of Erythrophleum suaveolens leaves extract and its fungicidal attributes on Aspergillus flavus isolated from maize seeds. The leaves of E. suaveolens were collected from Gwagalada, Abuja, Nigeria, and subjected to soxhlet extraction with methanol and chloroform as solvents to determine the bioactive compounds in the leaves compared to water crude extract. Different crude concentrations of the plant extracts were added for the antifungal effect into wells within Aspergillus flavus swabbed in a potato dextrose agar plate to diffuse at room temperature for 2hrs and the plates were incubated at 28^oC for 48hrs before measuring the diameter of the inhibition zones. Tannins, alkaloid, phenols, Triterpenoids, saponin, and flavonoid were present in the plant with tannin, flavonoid, saponin, alkaloid, and phenol having a concentration of 2.49, 1.448, 2.69, 0.505 and 0.366 mg/g respectively. The water, chloroform, and methanol extracts had an inhibitory diameter zone of 10 ± 0.58, 3 ± 0.58, and 0 mm, respectively at p<0.05. This study showed the presence of some bioactive compounds in E.  suaveolens. Furthermore, water extract of the plant had higher antifungal potency on A. flavus isolated from maize seeds.

Keywords: Antifungal, Aspergillus flavus, Bioactive, Erythrophleum suaveolens, Phytochemicals

 

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